Présentation de Florent Joffre, Responsable Développement analytique à l’ITERG, lors du 105ème Congrès annuel de l’AOCS, San Antonio, Texas, Etats-Unis, 4 - 7 mai 2014
Poster présenté par Florent Joffre, Responsable Développement analytique à l’ITERG , 11th Euro Fed Lipid, Antalaya, Turquie, 27-30 octobre 2013
In 2011, Barocelli et al. showed that free 3-MCPD presented a higher toxicity than diesters of 3-MCPD (MCPD-E) in rats. In a recent report dealing with human exposure to 3-MCPD, the European Food Safety Authority does the assumption of equivalent oral bioavailability between free and bound 3-MCPD. However, to date, no study on bioavailability of MCPD-E has been performed. Our work focused on the lipase activity (in vitro two-step digestion model) and absorption (in vivo lymphatic duct fistula model) of MCPD-E. A 4-weeks daily intake study of MCPD-E in rats gives information concerning theirs accumulations in organs.
Our results showed that MCPD-E are hydrolyzed by gastric and pancreatic lipases but the level of hydrolysis (close to 20%) is very low as compared to triglyceride (around 60-70% using the same two-step in vitro digestion model). This result was correlated however to the rate of absorption calculated in the lymphatic duct fistula model : only 25% of MCPD-E were absorbed after 24h. Finally feeding MCPD-E induce an accumulation of MCPD-E in gonads, kidney, liver and more surprisingly in heart.
In conclusion, it appears that MCPD-E were partially digested and absorbed and then accumulated in different target organs. Females had higher rates of accumulation that may explain the greater damage observed in toxicological studies.
Florent Joffre (ITERG, Pessac, France), Sawsan Amara (EIPL, Marseille, France), Frédéric Carrière (EIPL, Marseille, France), Leslie Couëdelo (ITERG, Pessac, France) et Carole Vaysse (ITERG, Pessac, France)