Poster présenté par Leslie Couëdelo, Responsable projets Nutrition, Métabolisme & Santé à l’ITERG, lors de la 57ème conférence ICBL, 4-8 septembre 2016, Chamonix
Leslie Couëdelo, Anthony Sehl, Aurélie Termon, Laurence Fonseca, Carole Vaysse
Background : Dietary flaxseed may have significant health-related benefits due to its high content of alpha-linolenic acid (ALA, 18:3 n-3). However, epidemiological studies have demonstrated an insufficient ALA consumption in western countries. Thus, improving ALA bioavailability without increasing lipid intake is a recognized nutritional challenge. Furthermore our previous work has shown that the emulsification with phospholipids (PL) would increase the bioavailability of ALA.
Aim : The objective of this study was to define, in rats, the impact of the interface creation and / or the presence of polar lipids (PL) on the omega-3 plasma bioavailability during postprandial period and the clearance of chylomicrons associated with LPL activity
Methods : After a Wash-out period, wistar adult male rats (n = 20 rats/group) were fed by gastric intubation with one of three experimental formulas 1) flaxseed oil 2) flaxseed oil not emulsified, added to 8% soybean lecithin, 3) flaxseed oil emulsified with 8% soybean lecithin. The ALA intake was similar between all experimental groups. Blood was collected at 1 h intervals for the next 5 hours post feeding.
Results and Discussion : Data showed that lipemia was higher in the group associating the PL, during the postprandial kinetics, regardless of the emulsification process. Moreover, the kinetics of lipids appearance in plasma was less diffuse, in the presence of PL. The increase in postprandial lipemia (p = 0.055) and the plasma concentration of ALA in the TG (p = 0.09) is even more pronounced, when PL were added in the flaxseed oil.
Plasma lipoprotein profile suggests that the CM were larger and more numerous in the group fed with the emulsified oil. These data are consistent with LPL activity which tends to be more important for this group, especially when the CM are large.